Association between CIRP expression and hypoxic-ischemic brain injury in neonatal rats
Identifieur interne : 000A59 ( Main/Exploration ); précédent : 000A58; suivant : 000A60Association between CIRP expression and hypoxic-ischemic brain injury in neonatal rats
Auteurs : Lifang Chen [République populaire de Chine] ; Qiaohuan Tian [République populaire de Chine] ; Weihua Wang [République populaire de Chine]Source :
- Experimental and Therapeutic Medicine [ 1792-0981 ] ; 2019.
Abstract
The role of cold inducible RNA-binding protein (CIRP) in mediating ischemic brain injury in neonatal rats under chronic hypobaric hypoxia was investigated. The neonatal rat model of chronic hypobaric hypoxia and the cell culture model of SH-SY5Y cells exposed to hypoxia (1% O2) were constructed. The expression of CIRP and hypoxia-inducible factor-1α (HIF-1α) was detected after hypoxic exposure, and the apoptosis-related proteins were analyzed via terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) and western blot analysis to detect neuronal apoptosis. Moreover, the effects of CIRP overexpression on HIF-1α and neuronal apoptosis were identified. Chronic hypobaric hypoxia can lead to HIF-1α expression and neuronal apoptosis in the body. CIRP was induced at early exposure (3 d/7 d). However, the CIRP level in the hypoxic group was obviously lower than that in the control group with the prolongation of exposure time (21 d). In addition, the knockdown of HIF-1α significantly reduced the neuronal apoptosis under hypoxic conditions, indicating that HIF-1α may promote apoptosis during exposure. The overexpression of CIRP significantly inhibited the upregulation of HIF-1α during hypoxia and the HIF-1α-mediated neuronal apoptosis. Results of the current study showed that, CIRP is involved in the ischemic brain injury induced by chronic hypoxia through downregulation of HIF-1α expression.
Url:
DOI: 10.3892/etm.2019.7767
PubMed: 31410103
PubMed Central: 6676150
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Association between CIRP expression and hypoxic-ischemic brain injury in neonatal rats</title><author><name sortKey="Chen, Lifang" sort="Chen, Lifang" uniqKey="Chen L" first="Lifang" last="Chen">Lifang Chen</name><affiliation wicri:level="1"><nlm:aff id="af1-etm-0-0-7767">Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000, P.R. China</nlm:aff><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000</wicri:regionArea><wicri:noRegion>Shandong 252000</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Qiaohuan" sort="Tian, Qiaohuan" uniqKey="Tian Q" first="Qiaohuan" last="Tian">Qiaohuan Tian</name><affiliation wicri:level="1"><nlm:aff id="af1-etm-0-0-7767">Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000, P.R. China</nlm:aff><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000</wicri:regionArea><wicri:noRegion>Shandong 252000</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Weihua" sort="Wang, Weihua" uniqKey="Wang W" first="Weihua" last="Wang">Weihua Wang</name><affiliation wicri:level="1"><nlm:aff id="af2-etm-0-0-7767">Department of Paediatrics, The First People's Hospital of Xianyang City, Xianyang, Shaanxi 712000, P.R. China</nlm:aff><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, The First People's Hospital of Xianyang City, Xianyang, Shaanxi 712000</wicri:regionArea><wicri:noRegion>Shaanxi 712000</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31410103</idno><idno type="pmc">6676150</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676150</idno><idno type="RBID">PMC:6676150</idno><idno type="doi">10.3892/etm.2019.7767</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000516</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000516</idno><idno type="wicri:Area/Pmc/Curation">000516</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000516</idno><idno type="wicri:Area/Pmc/Checkpoint">000989</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000989</idno><idno type="wicri:Area/Ncbi/Merge">000909</idno><idno type="wicri:Area/Ncbi/Curation">000909</idno><idno type="wicri:Area/Ncbi/Checkpoint">000909</idno><idno type="wicri:doubleKey">1792-0981:2019:Chen L:association:between:cirp</idno><idno type="wicri:Area/Main/Merge">000A59</idno><idno type="wicri:Area/Main/Curation">000A59</idno><idno type="wicri:Area/Main/Exploration">000A59</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Association between CIRP expression and hypoxic-ischemic brain injury in neonatal rats</title><author><name sortKey="Chen, Lifang" sort="Chen, Lifang" uniqKey="Chen L" first="Lifang" last="Chen">Lifang Chen</name><affiliation wicri:level="1"><nlm:aff id="af1-etm-0-0-7767">Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000, P.R. China</nlm:aff><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000</wicri:regionArea><wicri:noRegion>Shandong 252000</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Qiaohuan" sort="Tian, Qiaohuan" uniqKey="Tian Q" first="Qiaohuan" last="Tian">Qiaohuan Tian</name><affiliation wicri:level="1"><nlm:aff id="af1-etm-0-0-7767">Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000, P.R. China</nlm:aff><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, Liaocheng Third People's Hospital, Liaocheng, Shandong 252000</wicri:regionArea><wicri:noRegion>Shandong 252000</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Weihua" sort="Wang, Weihua" uniqKey="Wang W" first="Weihua" last="Wang">Weihua Wang</name><affiliation wicri:level="1"><nlm:aff id="af2-etm-0-0-7767">Department of Paediatrics, The First People's Hospital of Xianyang City, Xianyang, Shaanxi 712000, P.R. China</nlm:aff><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, The First People's Hospital of Xianyang City, Xianyang, Shaanxi 712000</wicri:regionArea><wicri:noRegion>Shaanxi 712000</wicri:noRegion></affiliation></author></analytic><series><title level="j">Experimental and Therapeutic Medicine</title><idno type="ISSN">1792-0981</idno><idno type="eISSN">1792-1015</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The role of cold inducible RNA-binding protein (CIRP) in mediating ischemic brain injury in neonatal rats under chronic hypobaric hypoxia was investigated. The neonatal rat model of chronic hypobaric hypoxia and the cell culture model of SH-SY5Y cells exposed to hypoxia (1% O<sub>2</sub>) were constructed. The expression of CIRP and hypoxia-inducible factor-1α (HIF-1α) was detected after hypoxic exposure, and the apoptosis-related proteins were analyzed via terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) and western blot analysis to detect neuronal apoptosis. Moreover, the effects of CIRP overexpression on HIF-1α and neuronal apoptosis were identified. Chronic hypobaric hypoxia can lead to HIF-1α expression and neuronal apoptosis in the body. CIRP was induced at early exposure (3 d/7 d). However, the CIRP level in the hypoxic group was obviously lower than that in the control group with the prolongation of exposure time (21 d). In addition, the knockdown of HIF-1α significantly reduced the neuronal apoptosis under hypoxic conditions, indicating that HIF-1α may promote apoptosis during exposure. The overexpression of CIRP significantly inhibited the upregulation of HIF-1α during hypoxia and the HIF-1α-mediated neuronal apoptosis. Results of the current study showed that, CIRP is involved in the ischemic brain injury induced by chronic hypoxia through downregulation of HIF-1α expression.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Maiti, P" uniqKey="Maiti P">P Maiti</name></author><author><name sortKey="Singh, Sb" uniqKey="Singh S">SB Singh</name></author><author><name sortKey="Muthuraju, S" uniqKey="Muthuraju S">S Muthuraju</name></author><author><name sortKey="Veleri, S" uniqKey="Veleri S">S Veleri</name></author><author><name sortKey="Ilavazhagan, G" uniqKey="Ilavazhagan G">G Ilavazhagan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, F" uniqKey="Pan F">F Pan</name></author><author><name sortKey="Zarate, J" uniqKey="Zarate J">J Zarate</name></author><author><name sortKey="Choudhury, A" uniqKey="Choudhury A">A Choudhury</name></author><author><name sortKey="Rupprecht, R" uniqKey="Rupprecht R">R 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